pfizer selective enterococcus agar  (Merck & Co)

Journal: bioRxiv

Article Title: Contact dependent suppression of Clostridioides difficile sporulation by enterococci requires the endocarditis and biofilm associated pilus

doi: 10.64898/2026.04.22.718763

Figure Lengend Snippet: (A-D) Monoculture and coculture of C. difficile in liquid SMC anaerobically for 48 hours with E. feacalis OG1RF , Enterococcus durans, Enterooccus faecalis, Enterococus faeicum, or Enterococus hirae followed by plating on selective media. n = 3-11 biological replicates combined from 4 independent expeirments. (A) Enterococci CFUs (B) Total C. difficile CFUs (C) Heat-resistant C. difficile spore CFUs (D) C. difficile sporulation efficiency. (E-H) Monoculture and coculture of Enterococcus saccharolyticus and C. difficile in liquid SMC anerobically for 48 hours followed by plating on selective medium. n = 8-9 biological repicates combined from 3 independent experiments (E) E. saccharolyticus CFUs. (F) Total C. difficile CFUs. ( G) Heat-resistant C. difficile spore CFUs. (H) Percent sporulation efficieincy. Statistics in A, E, F, G, H: t-test with Welch’s correction, statistics in B, C, D: Kruskal-Wallis One-way ANOVA with Dunn’s multiple comparisions test. LoD = Limit of Detection

Article Snippet: Samples inoculated in the well were plated on both BHI-CC-TA and Pfizer Selective Enterococcus Agar to confirm no bacteria migrated through the insert filter.

Techniques:

Journal: bioRxiv

Article Title: Contact dependent suppression of Clostridioides difficile sporulation by enterococci requires the endocarditis and biofilm associated pilus

doi: 10.64898/2026.04.22.718763

Figure Lengend Snippet: A-D) C. difficile and VRE were inoculated in transwells as indicated in the diagram on each x-axis. V = VRE, C = C. difficile. Transwells were incubated for 48 hours and plated on selective media. A) Total VRE CFUs. B) Total C. difficile CFUs. C) Heat resistant C. difficile spore CFUs. D) Percent sporulation efficiency. n = 6 biological replicates. Statistics: Pairwise comparisons for the effects of the presence and absence of a transwell: Unpaired Welch’s t-test. Multiple comparisons for differences in CFUs across conditions: Kruskall-Wallace One-way ANOVA with Dunn’s correction. E-G) Macrocolony migration assay, spots were inoculated on BHI agar and were incubated for 5 days followed by sampling the inside and periphery of the colony and selective plating. CD = C. difficile , 43076 = E. saccarolyticus E) Enterococcus CFUs, F) Total C. difficile CFUs, G) C. difficile heat resistant spore CFUs, H) Percent sporulation efficiency. Each data point represents one biological replicate combined from 3 experiments. Statistics: Kruskall-Wallace One-way ANOVA with Dunn’s correction. I) Representative images of 5-day single and dual species macrocolonies.

Article Snippet: Samples inoculated in the well were plated on both BHI-CC-TA and Pfizer Selective Enterococcus Agar to confirm no bacteria migrated through the insert filter.

Techniques: Incubation, Migration, Sampling